Isolation and identification of genes in staphylococcus aureus from panton valentine leukocidin dairy in mazandaran province

Pooran Pourramezanynejad,1 Sheyda ghasemi,2 Ali khosravi,3,*

1. Islamic Azad University of Ayatollah Amoli
2. Islamic Azad University of Ayatollah Amoli
3. Islamic Azad University of Ayatollah Amoli



The most important factors of food poisoning staphylococcus aureus infections in the world that has been in effect contaminated food. staphylococcus aureus that contains the gene for virulence panton valentine leukocidin (pvl) finally getting liz leukocytes and cause tissue necrosis. the aim of the present study, isolation and identification of staphylococcus aureus microbe pvl gene in dairy samples using pcr techniques.


This study was carried out in the year 2016 in which r 55 examples of traditional dairy products (milk and cheese, traditional) was collected at the level of the province. for the identification and isolation of staphylococcus aureus infections, bio-chemical, differential tests. to identify pvl gene pcr test was used.


Of the 55 samples tested, 20 samples (36.36%) with the laboratory techniques, pollution with staphylococcus aureus showed, that the 15 samples of milk and cheese from 5 samples were pvl gene in isolation none of the isolates of staphylococcus aureus identification.


Based on the results of this study because of the possibility of the presence of the bacterium staphylococcus aureus in milk and cheese to traditional, falling consumer health due to the presence of toxins and various enzymes in the production and distribution of bacteria, they should be under the control of the relevant professionals.


Staphylococcus aureus, panton valentine leukocidin, traditional dairy products, pcr