The effects of lgk974 and aspirin drugs on gene expression cox-2 in colorectal cancer cells
,1 Amirreza hesari
,2 Parisa ziasarabi
,3 Faezeh ghasemi
1. 1- Molecular and Medicine Research Center, Department of Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
2. 1- Student Research Committee, Faculty of Medicine, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
3. 1- Student Research Committee, Faculty of Medicine, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
4. 4- Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine,Next to Milad To
Colorectal cancer (crc) remains a major public health problem worldwide. regarding cyclooxygenase -2 (cox- 2) overexpression in most crc tissue and is associated with processes such as angiogenesis, inhibition of apoptosis, and metastasis of colon cancer cells, we have examined the effects of lgk974 and aspirin drugs on cox- 2 in sw742 colorectal cancer cells compared to oxaliplatin drug in vitro.
Mtt (methyl tetrazolium assay) colorimetric assay was used to investigate cell viability in the presence of drugs. real time pcr was used for the measurement of the effect of the drugs in cox-2 gene expression. statistical analysis was determined by one-way anova and tukey multiple range analysis (spss 19.0 software (p <0.05).
The analysis of the results indicated that treatment with lgk974 and aspirin drugs inhibited the growth of sw742 cancer cells. also, in this cell line, treatment with ic50 concentration of lgk974 and aspirin decreased expression of the cox-2 gene by 1.52 and 1.93 log fold change respectively.
We conclude that lgk974 and aspirin drugs cause inhibition of growth and decreases expression of the cox-2 gene in the sw742 cancer cells in vitro. so, these drugs might serve as a new strategy for the treatment of crc.
Aspirin, lgk974, colorectal cancer, sw742