The effects of a single nucleotide polymorphism in oatp2 gene on susceptibility to hyperbilirubinemia in an iranian neonates
Hassan Mehrad-majd,
1,* Abbas boskabadi,
2 Fatemeh khatami,
3 Gholamali mamouri,
4
1. Clinical Research Unit, Mashhad University of Medical Sciences, Mashhad, Iran
2. Department of Pediatrics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3. Department of Pediatrics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
4. Department of Pediatrics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract
Introduction
several genetic analyses have clearly linked a number of single nucleotide polymorphisms (snps) with idiopathic hyperbilirubinemia in newborns. recently it has been proposed that the variation in the oatp2 gene may contribute in neonatal hyperbilirubinemia. the present study was undertaken to explore the correlation of oatp2 rs2306283 polymorphism with the risk of hyperbilirubinemia in an iranian neonates.
Methods
Whole blood samples of the enrolled infants were collected and applied for dna extraction. genotypes for the oatp2 rs2306283 (388 a ➔ g) polymorphism were detected by polymerase chain reaction combined with restriction enzyme analysis (pcr-rflp) in 100 infants with hyperbilirubinemia and 100 controls. the genotypes and allele frequencies of the polymorphism were compared in each group.
Results
The genotypes and allele frequencies of the rs2306283 variant exhibited no significant association with neonatal hyperbilirubinemia neither in the crud state nor after adjustment for gestational age, gender and birth weight in different genetic models (p > 0.05).
Conclusion
The results indicated that oatp2 rs2306283 (388 a ➔ g) polymorphism does not appear to markedly play a role in the susceptibility to develop idiopathic hyperbilirubinemia among iranian neonates
Keywords
Oatp2, hyperbilirubinemia, polymorphism, neonates
