The effect of propolis treatment on interleukin 10 expression and arthritis severity in balb/c mice with collagen induced arthritis (cia)

Zahra Harsani,1,* Fatemeh rohollah,2



Rheumatoid arthritis (ra) is an inflammatory systemic disease that affects the joints, tissues, muscles, tendons, and fibrous tissues. the prevalence of rheumatoid arthritis is between 0.3% and 1%, and is often seen in developed countries and the female population. this disease is associated with progressive joint damage. in the development of inflammatory diseases, including rheumatoid arthritis, there are large molecular factors involved. among these factors, cytokines play a key role. proinflammatory cytokines such as interleukin 1 and tnf have been considered as important factors in the progression of the disease of rheumatoid arthritis. functionally, another group of cytokines has their role in modulating the immune system and inhibiting inflammation. interleukin 10 is a cytokine that inhibits inflammation and has been shown in several studies to modify its effects on the molecular flares of inflammation. propolis is a natural compound that exhibits various biological activities due to the high concentrations of flavonoids, including phenolic acid. based on the results of recent studies, experimental animals treated with propolis lead to changes in their immune system activity. based on recent findings, propolis administration results in the improvement of arteritis-induced inflammatory symptoms in laboratory animal. the main hypothesis in the present study is that the propolis extract may be induced by the expression of interleukin 10 as an anti-inflammatory mediator, leading to the improvement of arthritis-inflammatory symptoms. therefore, the effect of propolis on interleukin 10 expression and arrhythmic clinical findings in balb / c mice was investigated in this study.


Balb / c mice entered this study. at first, the mice were divided into 10 groups of 4, and in each group animals received a different type of collagen type ii injection, and this method induced a different degree of arthritis in each group. also, some groups of mice received a dose of 6.6 mg / g of food intake from propolis, and some groups used ordinary foods. the animals were treated with propolis for 36 days. after completing the treatment, interleukin 10 expression level at both levels of mrna and protein was measured and compared using real-time pcr and elisa methods in different animal groups.


Interleukin 10 has a significant relationship with propolis extract. propolis treatment results in increased expression of interleukin 10 at both levels of mrna and protein.


The results of this study indicate that propolis consumption for 36 days results in relief of arthritis symptoms and lesions in laboratory animals. propolis may have its anti-inflammatory effect by inducing interleukin 10 expression.


Propolis, arthritis, inflammation, interleukin 10