Comparison of different sources of mesenchymal stem cells for cell therapy in genomics aspects

Negar Ranjbaran,1 Fatemeh khatami,2 Mehrdad hashemi,3 Babak arjmand,4,*

1. ِِDepartment of genetics,Tehran Medical Sciences , Islamic Azad University , Tehran, Iran
2. Chronic Disease Research Center,Endocrinology and Metabolism Population Sciences Institute ,Tehran University of Medical Sciences, Tehran, Iran
3. Department of genetics,Tehran Medical Sciences , Islamic Azad University , Tehran, Iran
4. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran Metabolomics and Genomics Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Science,Tehran,Iran

Abstract

Introduction

Countries are disquiet about dying from the disease.cancer, diabetes, road injury are the most common diseases that expressed by the world health organization (who) as the top ten causes of death in the world.they have common side effects including amputation, chronic ulcers, face and body deformities.when these occur, more time and more costs of treatment are needed. cell therapy is new therapeutic approach for treatment. cell therapists utilize mesenchymal stem cells that are isolated from different tissues of the body in cell therapy.considering the ethical concerns and invasive methods of isolation, selecting source of mesenchymal stem cells that easily isolated, contains large number of cells, and also can be waste part of surgery attracted attentions.

Methods

Mesenchymal stem cells were isolated from fresh tissues of body. cell surface markers examine by flow cytometry for cd105, cd90, cd73, cd34, and hla-dr. specific culture mediums induce differentiation of mscs into adipocytes, osteocytes, and chondrocytes. quantitative real-time polymerase chain reaction used to identify gene expressions.

Results

Comparison between cell lines shows that they display similar spindle shape and cell surface markers were positive for cd105, cd90, cd73 and negative for cd34, hla-dr.we also investigated the differentiation potential and their gene expression.

Conclusion

These data will help pundits to choose appropriate source of mesenchymal stem cells for clinical application.

Keywords

Cell therapy, differentiation, gene expression, mesenchymal stem cell