Evaluation of relationship between age and nrf2 gene expression in alzheimers disease model of drosophila melanogaster

Javad Amini,1 Nima sanadgol,2 Mohsen shahriari,3 Mohammd hadadi,4 Naser sanchooli,5,*

2. department of biology, faculty of sience, university of zabol, zabol. iran



Alzheimers disease is the most common form of dementia among the aging. increasing the age is one of the risk factor for alzheimers disease, beta-amyloid extracellular plaques and neurofibrillary tangles are two main symptoms of alzheimers disease, which hyperphosphorylated tau protein pools can be reason of neurofibrillary tangles. one of the important events in alzheimers disease is oxidative stress in neurons. nrf2 is a transcription factor that is expressed in response to low active oxygen levels, which can regulate the antioxidant response, hence it can be a good indicator of oxidative stress.


Because drosophila melanogaster has a short life cycle, maintaining a convenient and inexpensive is a very good model for neuronal studies, so we use this model. the lines of usa-tau r406w and gal4-elav, gal4-ok 107, gal4-gmr were prepared. we isolated female virgin fly from gal4-elav and cross with male uas-tau r406w and then we extract rna from progeny at 10 days, 20 days and 30 days and we quantify with qreal-time pcr. also, we cross female virgin from gal4-ok107 and male uas-tau r406w and use for behavioral assay at 10 days, 20 days and 30 days flies. we setup cross between gmr-gal4 and uas-tau r406w for the tau r406w expression conformation.


As a result, over expressing the tau r406w protein in drosophila melanogaster neurons, with increasing age, the expression of nrf2 increased, and decreased the memory in flies efficiently.


Age increasing due to the over expression of nrf2, indicating increased oxidative stress levels with age in the alzheimers model.


Alzheimers disease, nrf2, tau r406w, drosophila melanogaster