Evaluation of cytotoxicity effects and nm23 gene expression against breast cancer t47d cells treated by zn oxide nanoparticles( znnps)

Fatemeh Ghodratpor,1,* Fahimeh baghbani-arani,2 Seyed ataollah sadat shandiz,3

1. Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University
2. Assistant Professor, Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch
3. Assistant Professor, Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction

Breast cancer is the second leading cause of cancer related death worldwide among women. many investigations have confirmed that the nm23 gene suppresses metastasis in various types of cancers. zinc oxide nanoparticles (znnps) are one of the most useful mineral particles considering its high chemical stability, low dielectric constant, high catalytic activity, anti bacterial and anti cancer properties. the aim of this study was to investigate the cytotoxic activity of znnps on breast cancer t47d cell line and to evaluate nm23 gene expression in treated cells by mentioned nanoparticles.

Methods

The cancer t47d and normal hek293 cell lines were treated with different concentration of znnps for 48 hours and the cytotoxicity activity of znnps was calculated by colorimetric mtt assay. then, nm23 compared to gapdh reference gene expression was evaluated using real time pcr technique.

Results

Based on the mtt results, the cytotoxic activity of znnps has dose- dependent manner against t47d cell lines. on the other hand, nm23/gapdh gene expression ratios were 7.8 (p<0.05) for ic50 concentration of znnps.

Conclusion

Based on the current obtained data, it seems that the znnps can modulate metastasis by enhancing the nm23 gene expression level in breast cancer t47d cells and further clinical studies on animal model and other cancer cell lines are required.

Keywords

Breast cancer, znnps, cytotoxicity, metastatic, nm23.