Alterations of gyra in enterobacteriaceae isolated of urinary tract infection resistance to quinolones antibiotics

Atousa Monavari,1,*



Urinary tract infection is one of the most common infections, which if not treated, it can be cause seriouse problem in patients. one of the ways to treat of this infection is antibiotic therapy. nowaday antibiotic resistant in microorganisms is a main problem for physicians and patients in the world. the aim of this study is to evaluate the genetic resistance to quinolones antibiotics in enterobacteriaceae isolated of urine samples.


: initially suspected sample of urinary infection were collected.after isolation, diagnostic tests performed for enterobacteriaceae family, for evaluation of antibiotic suscebtibity in bacteria antibiogram test performed by quinolone antibiotics. later dna was extracted and pcr was performed by using specific primers of gyra gene.


Hundred bacteria were isolated of clinical urine sample including 60 e.coli, 32 klebsiella, 3 enterobacter, and 5 proteus. bacterial antibiotic resistance to ciprofloxacin were 36%, nalidixic acid 45%, norfloxacin,ofloxacin 38%, levofloxacin 35% and enrofloaxin 39%. in overall 36 bacteria were resist to all antibiotics, which 29 bacteria(80.55%) revealed mutation in gyra gene.


This study revealed that ecoli isolates carry a mutation in gyra genes. this mutations have an important role in antibiotic resistance to quinolons.


Antibiotics resistan