1. Islamic Azad University, Zanjan Branch, Faculty of Baisic sciences, Department of Genetics, Etemadeyeh, Moalem St. 2. Islamic Azad University, Zanjan Branch, Faculty of Baisic sciences, Department of Genetics, Etemadeyeh, Moalem St.
Claudins are the main structural and functional proteins of tight junctions in epithelial cells. the altered expression patterns of differ¬ent claudin members have been demonstrated in a variety of diseases, particularly in cancers. it has been reported that claudin 1 is overexpressed 22-fold in gastric adenocarcinoma. considering the least side effect of herbal extracts, in this study we have evaluated claudin-1 gene expression in ags cells treated with ziziphora extract.
The ags cells were incubated 37°c containing 5% co2 with 85% humidity dmem with 10% fbs . the cells were treated with concentrations of 800, 1200, 2000 μg /ml of ziziphora for 48 hours. extraction of rna, synthesis of cdna has been done using kit. the study of claudin-1 gene expression was performed by real time pcr and also gapdh gene was used as the internal control.
The results of real time pcr data have shown reduction of 7 and 2.3 fold with concentrations of 800 and 1200 μg /ml of ziziphora respectively and there was no significant change in concentration of 2000μg / ml .
Claudin-1 may become therapeutic targets for cancer treatment and ziziphora as a herbal extract decreased the expression rate of claudin1significantly, in lower concentrations treatment. it seems that this herbal extract has tremendous anticancer effects.