Mir-133 : a prognostic and diagnostic biomarker for breast cancer patients

Rezvan Mohammadi,1 Amirreza hesari,2 Soheila moeini,3 Faezeh ghasemi,4 Reza salarinia,5,* Somaye noruzi,6

1. MSc student of Medical Biotechnology, School of Medicine, Northern Khorasan University of Medical Sciences, Northern Kh
2. Department of Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
3. Mashhad University of Medical Sciences, Mashhad, Iran
4. . Department of Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
5. Department of Medical Biotechnology and Molecular Sciences, School of Medicine, North Khorasan University of Medical Sci



Breast cancer is known as one of major malignancies which could initiation from breast tissue. this cancer is found as the most common cancer associated death among women worldwide. micrornas (mirnas) are epigenetic regulators which have critical roles in modulating of various biological processes such as angiogenesis, growth, and differentiation. multiple lines of evidence indicated that deregulation of them could be associated with initiation and progression of various cancers such as breast cancer. among of various mirnas, mir-133 could has critical roles in breast cancer pathogenesis. in the current study, we investigated the clinic-pathological roles of mir-133 in breast cancer.


Total rna was extracted from serum of 100 patients with breast cancer .cdna of micro rna 133 and u6 was synthesized with stem loop primers. sybr green qpcr was performed to measure mir133 and u6 . u6 was used as an internal control . rna expression levels were obtained the comparative cycle threshold 2−ΔΔct method and analyzed as mean ± sd.


The expression of mir-133 was remarkably decreased in serum of breast cancer patients than control group (p <0.05).


The expression levels of mir-133 were down regulated in breast cancer patients. the down regulation of mir-133 was associated with lymph nodes metastasis, high clinical stages and shorter relapse-free survivals of patients with breast cancer. our results demonstrated that expression levels of mir-133 expression could be employed as prognostic and diagnostic biomarkers for patients with breast cancer.


Breast cancer, mir-133, biomarker