Curcumin regulates proinflammatory signaling function of thrombin in endothelial cells
Methods
Huvec isolated and cultured in the presence and absence of curcumin (10μmol/l) for 1, 3 and 5 hours followed by incubation with thrombin (40 nm). then total rna was extracted, cdna was synthesized and the expression of inflammatory hsps including hsp60 were analyzed by real time pcr.
Results
The results of this research showed that treatment of huvecs with thrombin significantly increased expression of inflammatory hsps including hsps60 compared to non-treated huvecs and this effect was entirely suppressed by curcumin.
Conclusion
As a result, thrombin increases inflammatory hsps expression and triggers inflammatory mechanisms. curcumin as an anti-inflammatory molecule reduces thrombin-induced proinflammatory signaling responses. it is suggested that curcumin is an attractive candidate target for therapeutic intervention in thrombin-associated inflammatory disorders including cancer, cardiovascular disease, and metabolic syndrome.
