Chronic myeloid leukemia is a cancer of blood which is identified by bcr/abl translocation. imatinib is a tyrosine kinase inhibitors and is affective in treatment of this disease. sirt1 is a gene with deacetylase activity which has been detected to have increased expression in many cancers. the aim of this study was to evaluate the relationship between iron nanoparticle and imatinib on the expression of sirt1 gene in k562 by real time pcr in laboratory condition.
Methods
For this purpose, k562 cell line was prepared from pasture institute and cultured in rpmi enriched with fetal calf serum and co2 at 37 ºc. then the viability was measured by trypan blue and cell counted and divided into four groups. one group selected as control (without any treatment), group second to fourth treated with iron nanoparticle, imatinib and a combination of iron nanoparticles and imatinib. after 72 h the expression of sirt1 gene was assayed by real time-pcr.
Results
Results showed that 200 µg/ml of zinc nanoparticle could significantly (p=0.024) inhibit the growth of k562 cell lie. also abstained finding for real time pcr documented that 200 µg/ml of zinc nanoparticle could increase the expression of p53 gene than he control but it was not significant
Conclusion
It’s concluded that zinc nanoparticle could inhibit the growth of k562 and p53 expression, but more study should be done for obtain he bet results.
Keywords
K562 cell line, p53 gene, real time pcr, zinc nanoparticle.
