Lactococcus lactis is known as probiotics due to its lack of harmful toxins. because of its high growth potential and resistance to acidity loss and digestive proliferation as an oral drug, these advantages have led to the idea of a heterologous protein production platform organic. in this study, chimeric antimicrobial peptide lactoferricin-lactoferampin in l.lactis was synthesized. thus, by synthesizing this peptide in l.lactis, we benefit from the antimicrobial function of this combination of chimera and the probiotic benefits that it produces. in this study, the production of e resulted in the superiority of the antimicrobial activity of this chimeric compound and the probiotic benefits that it produces together. we will benefit
Methods
The codon optimization and addition of restriction sites and the codon of the beginning and the end and the synthesis of the nucleotide sequencing were ordered. subsequently, the vector was cleaved with sali, sapi enzymes and extracted from the gel and then the vector was cut with the same enzymes and the ligation reaction, and we made the recombinant vector. sub clones in e.coli and extraction of plasmid with electroporation to amj 1543 in strain amj 1543 in a non-alanine medium. selection of recombinant media was carried out by recombinant lactococcus lactis culture and collecting the culture medium first, confirming the expression and secretion on the sds gel and finally testing the diffusion disk on the 4th pathogen was a food index.
Results
Confirmation of ligation reaction with pcr colony was performed with corresponding vector primers, confirmation of expression and secretion with kd 4 on sds gel and favorable antimicrobial activity was confirmed by increasing the halo diameter with increasing peptide level
Conclusion
.a clear perspective on the use of this peptide as an oral drug can be considered with the continuation of research.
