• Stereological investigation of the Androgenetic alopecia (AGA) after treatment with Minoxidil solution and conditioned media
  • AsiyeKamali1, 2,1,* Zahra Zamanzadeh,2 Mojgan Asadi,3 Majid Kamali,4 Negar Azarpira,5
    1. 1Department of Genetic, Faculty of Biological Sciences and Biotechnology, Shahid Ashrafi Esfahani University, Isfahan, Iran 2 Department of Tissue Engineering and Cell Therapy, School of Advanced Technologies in
    2. Department of Genetic, Faculty of Biological Sciences and Biotechnology, Shahid Ashrafi Esfahani University, Isfahan, Iran
    3. Department of Genetic, Faculty of Biological Sciences and Biotechnology, Shahid Ashrafi Esfahani University, Isfahan, Iran
    4. Department of Tissue Engineering and Cell Therapy, School of Advanced Technologies in Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
    5. Department of Tissue Engineering and Cell Therapy, School of Advanced Technologies in Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


  • Introduction: Male-pattern baldness, also known as androgenetic alopecia (AGA), is a dihydrotestosterone-mediated hair loss disorder that is characterized by a shortened anagen hair cycle that occurs when the follicles gradually shrink, resulting in thinning hair. The process of transforming thinning hair into thick hair is important in the development of successful treatment by stimulating and extending the length of the anagen phase, inducing and maintaining the anagen in the hair cycle. This study was carried out to investigate the effect of conditioned media derived from umbilical cord mesenchymal stem cells and minoxidil separately and simultaneously on male-pattern hair loss.
  • Methods: Testosterone dissolved in the thermo-sensitive gel based on poloxamer 407was injected into 4-weeks-old mice weighing 30 g to induce male pattern hair loss. Then, 5% minoxidil was applied topically daily, then the gel was optimally injected in two stages subcutaneously. Two months later, their skin was placed in formalin for stereology and H&E staining tests.
  • Results: Treatment of mice models with minoxidil made significant changes such as reduction of volume density of sebaceous glands and volume density of hair follicles compared to the model group, but these changes in treatment with conditioned media and minoxidil were more significant and effective.
  • Conclusion: Therefore, it was found that treatment with conditioned media could be a more effective method compared to other treatments such as minoxidil. Also, treatment with conditioned media has the same effect as treatment with conditioned media and minoxidil simultaneously.
  • Keywords: Androgenic Alopecia, Minoxidil, conditioned media