• Evaluation of gene expression of thymic stromal lymphopoietin (TSLP) in sputum of patients with persistent severe asthma
  • Mohammadreza Pourmohammad,1 Jina Khayatzadeh,2 Mohammadreza Khakzad,3,*
    1. Department of Biology, Faculty of Basic Sciences, Islamic Azad University
    2. Department of Biology, Faculty of Basic Sciences, Islamic Azad University
    3. Department of Immunology, Faculty of Medicine, Islamic Azad University


  • Introduction: Asthma, as a respiratory disease, is a chronic inflammatory complication of the all ways that causes narrowing of the airways and the development of sensitivity and resistance to air currents. This disease causes a lot of costs to the individual and the medical tem of the country every year. Also, because the population of asthmatics is increasing worldwide, thymic stromal lymphopoietin it is very important to study the factors that can aggravate the disease. The thymic stromal lymphopoietin gene is an important cytokine in the development of asthma and respiratory tract inflammation. This cytokine is expressed in various cells, including the airway epithelial cells. Many environmental factors such as allergens, germs, cigarette smoke, etc. can trigger the production of TSLP
  • Methods: In this study, TSLP gene expression in sputum of patients with stable severe asthma was investigated by RT-PCR. A total of 31 patients with asthma and 31 control samples were studied.
  • Results: The results showed that there was no significant difference between the control and asthma groups in terms of TSLP gene expression distribution. Also, a significant linear correlation was observed between TSLP gene expression and body mass index and asthma severity .the relationship between age, gender, cough and shortness of breath with gene expression intensity in people with severe asthma was not significant, but there was a significant difference between asthma severity and TSLP gene expression And as the severity of asthma increases, so does gene expression.
  • Conclusion: It seems that by suppressing the expression of TSLP or creating polymorphisms in this gene this disease can be reduced. It is recommended that other inflammatory cytokines affecting persistent severe asthma, which may make the disease more difficult to control, be investigated.
  • Keywords: severe Persistent asthma, Real Time, thymic stromal lymphopoietin (TSLP), Sputum