• Using CRISPR/Cas9a for Design guideRNA in human AML cells after treatment with SirTi and MC2494
  • Amir Gholamzad,1 Mahdi Nakhaee,2 Hadis Sadeghi,3 Mohammadmatin Nourikhani,4 Bita Ahmadi,5 Mehrdad Gholamzad,6,*
    1. Department of Laboratory Medicine, Faculty of Paramedical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    2. Department of Laboratory Medicine, Faculty of Paramedical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    3. Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    4. Department of Laboratory Medicine, Faculty of Paramedical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    5. Department of Laboratory Medicine, Faculty of Paramedical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    6. Department of Microbiology and Immunology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran


  • Introduction: Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy with regard to the pathogenetic mechanism, clinical phenotype, and treatment outcome(1).Despite current treatments, the clinical outcome of patients with AML is commonly poor(2). The CRISPR system has demonstrated unprecedented potential for treating cancer and genetic diseases through gene editing (3). Cas9 endonuclease is currently the most widely used CRISPR-associated nuclease (4).
  • Methods: Geo dataset GSE48295 downloaded from Geo datasets. Genes are compared with logfc (fold change) for their expression in MC2494 and SirTi group and then sorted by their p-value. Also genes function and ontology are identified by DAVID database. Finally genes with most modification and related to critical pathway in immune system are selected and their best gRNAs(guide RNA) in CRISPR system are identified for editing by CHOPCHOP webtool.
  • Results: SERPINB2 or serpin family B member2 has hyperexpression among MC2494 and SirTi group. It plays an important roles in immune system and inflammatory response. The best gRNAs for this gene is identified through CHOPCHOP database.
  • Conclusion: Many experiences showed that immune genes play an important roles in AML progression and metastasis, therefore they can be as a therapeutic target for CRISPR/Cas9 system and gene therapy.
  • Keywords: Many experiences showed that immune genes play an important roles in AML progression and metastasis,