The Effect of Nano-Chitosan Particles on Candida Biofilm Formation
The Effect of Nano-Chitosan Particles on Candida Biofilm Formation
Ali Asgharzadeh,1Ensieh Lotfali,2,*Mohammad Hossein Hajali,3Mahyar Keymaram,4
1. School of medicine, Aja University of Medical Sciences, Tehran, Iran. 2. Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 3. School of medicine, Aja University of Medical Sciences, Tehran, Iran 4. Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Introduction: Candida species are the most common pathogenic fungus and have the ability to form biofilms and represent an essential virulence factor. Candida biofilm formation is clinically important which can be the reservoirs of candidiasis. Nano-chitosan particles has an extensive antimicrobial scale against various pathogenic microorganisms. Nano-chitosan particles reveal eminent antibiofilm conducts versus multidrug-resistant pathogens and are contemplated a potential substitution to common drugs. The aim of this study was to evaluate the effect of nano-chitosan particles on candida biofilm formation.
Methods: In this in-vitro study, nano-chitosan particles were synthesized. These nanoparticles are approved by transmission electron microscope. Candida albicans were collected from the oral cavity of patients with oral candidiasis. Nano-chitosan particles with concentrations of 0, 1%, 10%, and 20% were exposure with suspension of Candida albicans isolates. Then biofilm formation was measured with crystal violate staining method.
Results: Nano-Chitosan Particles showed strong antimicrobial activity against Candida strain. The observed differences between untreated Candida strain (control) and treated Candida strain with nano-chitosan particles in terms of biofilm formation were significant (P<0.05).
Conclusion: The finding shows that nano-chitosan particles can have a significant impact on Candida biofilms. These substances are estimated to become conceivable candidates for the treatment of fungal disease caused by candida species due to their excessive effect on biofilm. However, cytotoxicity testing is also required.