Investigating the association between MiR199a-5p, miR-3120d and renal cancer in PI3K pathways
Investigating the association between MiR199a-5p, miR-3120d and renal cancer in PI3K pathways
Alireza Nasr Esfahani,1,*Setare Samizade,2
1. Department of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran 2. Department of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Introduction: Renal cell carcinoma (RCC) is the most common type (>80%) of kidney cancer.
Claiming more than 100,000 lives per year worldwide, RCC accounts for about 3% of all adult
cancers and its incidence is rising. With newer therapies, the median survival period of patients
with advanced RCC is about 26 months. MicroRNAs (miRNA), are small noncoding RNAs
that are emerging as important modulators in cellular pathways and appear to play a role in
tumorigenesis, cell cycle, proliferation and apoptosis. So we purpose to evaluate the role of
Ras, IKK, NFkB1, PI3K by mir-3120d, 199a-5p in renal cancer.
Methods: The feature of
microRNA was archived by using mirbase. Target genes and predict one were identified by
mirtarbase and mirwalk 2.0. Gene expression in normal and tumor tissue was obtained from
NCBI. Finally, the pathway enrichment analysis was performed by the KEGG and David.
GENEMANIA used to find gene network.
Results: The result dedicated that mir-199a-5p and
3120d by inhibiting Ras which actives Raf1, MEK, ERK through phosphorylation prevent
proliferation of cancer cell and angiogenesis. IKK, NFkB were inhibited by blocking PI3K,
which actives Ras. So MYC, BCL through inhibition cell survival, suppress cancer. Mir-199a5p, and 3120d by inhibiting Ras, PI3K, IKK, NFkB through preventing cell cycle, prevent
cancer development. Mentioned microRNAs, block NFkB by inhibiting PI3K which actives
Act. So NFkB by inhibiting VEGF, suppresses cancer through inhibition angiogenesis.
Conclusion: Mir-199a-5p, 3120d by effecting on Ras, IKK, NFkB1, PI3K, in PI3K-Act, RAS,
TNF and renal pathway effect on proliferation, angiogenesis and cell survival. Mentioned
microRNAs by acting as tumor suppressor prevent cancer development and tumor spread by
inhibiting proliferation of cancer cell, angiogenesis and sell survival.