expression analysis of INHBA mRNA in colorectal cancer and Integrated analysis of ceRNA network
expression analysis of INHBA mRNA in colorectal cancer and Integrated analysis of ceRNA network
Maryam Siahtiri,1,*Mohammad Rezaei,2Mansoureh Azadeh,3
1. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran 2. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran 3. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
Introduction: Colorectal cancer (CRC) is one of the most common cancers in the world. Multiple factors and bio-processes are associated with tumorigenesis and metastasis of CRC [1]. Competitive endogenous RNAs (ceRNAs) theory has revealed a new mechanism of interaction between RNAs (mRNAs, miRNAs, and lncRNAs) that is used to find the involved genes. In this study, we used microarrays to detail gene expression and target novel biomarkers for diagnosis and curing CRC.
Methods: Our study aimed to define the potential biomarkers in CRC via the analysis of a competitive endogenous RNA (ceRNA) network. Therefore, bioinformatics analysis targets novel biomarkers for diagnosis and curing CRC. First, NCBI Gene Expression Omnibus (GEO) was selected to get the desired GSE (GSE 25071 ), and therefore the gene expression profile was analyzed by GEO2R to search out differentially expressed genes (DEGs) in CRC tissue compared to controls, so the gene was selected for further studies. The most significant gene (|logFC| > 3 and p value< 0.05 ) were selected and Then was taken to miRWalk 3.0 [2] to find miRNA. We searched for our target miRNA in LncBace v.3 [2], and CASC19 was selected as a suitable lncRNA. Ultimately, the pathways that the gene was involved in were analyzed and studied to find that role in Cancer.
Results: Based on the microarray analysis that was carried out, INHBA had selected as significantly up-regulated mRNA (|logFC|=5/07 and adj p-value = 3.33E-10), and based on miRWalk interaction analysis mir-21-3p had a significant strong interaction with mRNA ( Score: 1, Energy: -24.1) and by using Kegg database pathways were examined individually [3]. INHBA is involved in Cytokine- cytokine receptor interaction and TGF-beta signaling pathways and signaling pathways regulating pluripotency of stem cells. In addition, mir-21-3p correlated with the most remarkable prognostic values of CRC patients [4], and this miRNA has RNA interaction with, CASC19 lncRNA.
Conclusion: Contemplating the above paragraphs, it is concluded that INHBA with mir-21-3p and its CeRNAs (competing endogenous RNAs) CASC19 act as a biological network with an important role in CRC. the mentioned lncRNA CASC19 expression is associated with tumor size, lymph node metastasis, and distant metastasis, suggesting high CASC19 expression may promote CRC metastasis [5]. the participation of INHBA in a CeRNA network, as well as the signaling pathway, demonstrated the possibility of INHBA being a reliable biomarker for diagnostic and prognostic goals.