Investigating the interaction between the BIRC5 gene as a biomarker and its associated miRNA in breast cancer through bioinformatics analysis
Investigating the interaction between the BIRC5 gene as a biomarker and its associated miRNA in breast cancer through bioinformatics analysis
mehdi roshanian bakhsh,1Zahra Ahmadzadeh Chaleshtori,2pegah javid,3Mansoureh Azadeh,4,*
1. Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran 2. Department of Biochemistry, falavarjan branch, Islamic Azad University, isfahan, Iran 3. Zist Fanavari Novin Biotechnology Department, State Technical and Vocational Training Organization, Isfahan, Iran 4. Zist Fanavari Novin Biotechnology Department, State Technical and Vocational Training Organization, Isfahan, Iran
Introduction: Almost every year, tens of thousands of women worldwide die from breast cancer (BC), one of the most aggressive malignant tumors. With about 17 million cases occurring annually, its prevalence is alarming. Bioinformatics analysis was conducted to identify the genes differentially expressed in carcinogenesis and progression of BC.
Methods: The GSE21422 was selected from GEO datasets to identify the differentially expressed genes (DEGs) between 5 tumor and 5 healthy samples in a breast cancer microarray experiment. The GEO2R analysis was performed on DEGs from BC samples compared to noncancerous samples. The adjusted P-value < 0.01 and log2FC (fold change) >5 were considered as statistical significances. Gene functional categories were determined by using the DAVID database as a Gene Functional Classification Tool. The analysis of the DEGs pathways was done in the Kyoto Encyclopedia of Genes and Genomes (KEGG). The associated microRNA (miR) with Baculoviral IAP Repeat Containing 5 (BIRC5) gene was found in the miRDB online database. The miRWalk online database was used to find the interactions between selected miR and associated genes.
Results: A total of 23 DEGs were identified, and GO analysis revealed the role of the BIRC5 gene in the anti-apoptosis of breast cancer. The KEGG database showed that the BIRC5 gene is a member of the inhibitor of apoptosis (IAP) gene family, which encodes negative regulatory proteins that prevent apoptotic cell death. The inhibition of this gene plays an essential role in the apoptosis of breast cancer. The results also showed that has-mir-548t-3p could have a suppressive impact on the inhibitory activity of the BIRC5 gene through apoptosis.
Conclusion: It was found that BIRC5 could act as a biomarker and play a role in anti-apoptotic processes in breast cancer cells based on bioinformatics research. The has-mir-548t-3p can be used as a treatment strategy to lead these cells to apoptosis in treating such patients.