Investigating the Co-culture of different types of PC12 cell lines on myogenic development of rat adipocyte-derived stem cells
Investigating the Co-culture of different types of PC12 cell lines on myogenic development of rat adipocyte-derived stem cells
Zeinab Shafiei Seifabadi,1Dian Dayer,2Vahid Bayati,3,*Seyed Saeed Azandeh,4Mohammad Rashno,5
1. Ph.D Student of Anatomical Sciences, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 2. PhD of Molecular Medicine, Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 3. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 4. Department of Anatomical Sciences, Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 5. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Introduction: Researchers are looking for a way to better the myogenic differentiation of stem cells. It has been found that various factors and conditions may increase the progenitor activity of adipose-derived stem cells (ADSC). In the current study, we tested whether PC12 cell lines could improve the ability of ADSCs to support the myogenesis.
Methods: Rat ADSCs were isolated, cultured in vitro, and identified using flow cytometry. Following that, purified ADSCs were co-cultured with three PC12 cell lines. MyoD mRNA expression was detected using real-time reverse-transcriptase polymerase chain reactions (Real-time RT-PCR) And the length of neurites of the PC12 cell lines was checked using ImageJ software.
Results: Our results reveal a significant difference in the mRNA expression of the MyoD gene between ADSCs and ADSCs and PC12 cell lines co-culture groups in myogenic differentiation culture medium (P<0.001) and PC12 cell lines effect improved expression of this gene. Current data revealed that the co-culture of ADSCs and PC12 cell lines could develop Long neurites formation in the co-culture group.
Conclusion: It was concluded that ADSCs/PC12 cell lines co-culture can lead to the formation of differentiated myoblasts in proximity to long neurites formations. These in vitro cell models can be used as basic research in Nero-muscle tissue engineering implanted into organ defects where muscle tissue and Neurological regeneration were required.