The effects of ZnO nanoparticles on the expression of TNF-α genes in infected Staphylococcus aureus Balb/C mice
The effects of ZnO nanoparticles on the expression of TNF-α genes in infected Staphylococcus aureus Balb/C mice
Yeghane Gholami,1Behnaz Esfandiari,2,*Javad Arasteh,3Amin Khalili,4
1. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran 2. Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran 3. Assistant Professor of Immunology, Islamic Azad University, Central Tehran Branch, Tehran, Iran 4. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Introduction: Methicillin - resistant Staphylococcus aureus strains are one of the most important clinical and epidemiological problems in hospitals. Currently, antibiotic resistance in bacteria is a major concern among physicians who are the main cause of treatment of patients and increased mortality. Nowadays, with the advancement of nanotechnology, nanoparticles have many capabilities in approaches to diagnosis, medical treatment, and especially drug delivery and gene therapy systems. The present study aimed to investigate the effect of ZnO nanoparticles on the expression of TNF-α genes in Balb/C mice infected with Staphylococcus aureus.
Methods: The microbial species used in this study was Staphylococcus aureus. The number of 35 Balb/C were purchased with 22 ± 5 g and all sexes and divided into five groups of seven. On the first day in the morning, the control and nano-control groups were injected intraperitoneally with saline so that the effect of shock induced by injection was similar in the groups. The third, fourth, and fifth groups received an intraperitoneal microbial suspension. Three hours later, the first control group received saline, the second group received nano-ZnO, the third group received saline, the fourth group received nano - ZnO, and the fifth group received vancomycin. Finally, after a 5 - day and 1- day period, samples of spleen preparation, RNA extraction, and Real-time were evaluated for TNF- α gene expression.
Results: The results of the study of different treatments with control samples indicated that at 5 days after vancomycin, the use of nano- ZnO can increase the expression of the TNF- α gene. While during the 1- day period just vancomycin TNF- α gene expression.
Conclusion: Finally, by examining the treatments, nano - ZnO was observed to increase the expression of TNF- α as compared to nano - copper and ZnO, which is the most significant effect of the gene. This shows its greater effect during the increase in the length of the treatment. Therefore, it can be concluded that nanoparticles can be used to replace common antibiotics against the aforementioned bacteria.