• Advantages of whole Genome Sequencing in Metagenomics Processes and DNA Biosynthetic Approach in Cancer
  • Alam Ara Gholami,1 Ali Ahmadi,2,*
    1. Assistant Professor, Department of Biological Sciences and Technologies, Islamic Azad University , Sari, Iran
    2. BSc. Student, Department of Biological Sciences and Technologies, Islamic Azad University Sari Branch, Sari, Iran


  • Introduction: Recent technological and innovative advances in integrated and continuous imaging, sequence-based measurements and computational analysis have transformed our view of genomes in terms of their structure and dynamics in space and time. These advances provide a deeper understanding of genome functions and mechanical insights into how the nucleus is organized and functions spatially. These factors provide us with a wide range of complementary data and the opportunity to produce measurable and integrated models of the nuclear organization. Next-generation sequencing (NGS) is widely used to diagnose rare genetic disorders. Large differences in the inclusion of cost and effectiveness parameters have been identified between studies. The validity and scale of the results can therefore be questioned, which has prevented valid comparisons and widespread generalizations. Comparable decisions are needed to implement new NGS-based diagnostic methods in pediatric genetics and beyond. The aim of this study was to determine the benefits of whole genome sequencing in metagenomic processes and DNA biosynthetic approach.
  • Methods: This study was a secondary study with a narrative approach approach that in 2022 by searching for keywords such as, sequencing, NGS, genome, metagenomics, heterolog and gene clusters in valid databases such as, Scopus , Sciences Direct, Web of Sciences and PubMed. All input and output criteria of the study were examined. In this study, 15 articles were selected, of which 10 articles were included in the study
  • Results: According to studies from various articles, reported diagnostic efficiencies ranging from 3 to 70%, with a higher range of efficiencies for neurological symptoms and acute illness from 22% to 68% and 37-70%, respectively. Became. Diagnoses cause a range of changes with a higher frequency for acute disease reported from 67 to 95%. The frequency of species of unknown importance varied from 5 to 85% between studies with the potential to decrease in frequency over time and the higher rates identified in patients with non-European ancestors. This study provides evidence for a higher range of the diagnostic efficiency of exome / genome sequencing compared to standard genetic testing, especially in neurological and acute symptoms. However it is essential to improve diagnostic accuracy and efficiency and to be able to analyze trends over time inhibits all Ca2+-dependent pathways in their signaling domain. Switched-off effectors/pathways are represented by white ellipses/arrows.
  • Conclusion: According to Results, genome sequencing has the potential for improvement and the process of clinical progression for patients undergoing genetic testing. Evidence of clinical utility is limited to the pediatric population.
  • Keywords: sequencing, NGS, genome, metagenomics, heterolog and gene clusters