Review : application of ngs and challenges related to dna typing of biological samples in legal genetics

Azam Soleimani,1,*

1. Legal Medicine Research Center, Legal Medicine Organization, Tehran,Iran.



During recent decades, development in molecular genetic related techniques have provided a hope for solving the challenges of genetic science. one of the most recent achievements is the next generation sequencing generation (ngs) technology. low levels of dna or the destruction of many of the samples referred to forensics resulted in unsuccessful function current capillary electrophoretic system for snps detection. therefore, any protocol and technology that covers this limitation is very helpful in solving a large number of legal cases. the purpose of this review is to evaluation the applications of ngs and the challenges associated with dna typing biologic samples in legal genetics.


Related studies from various databases were obtained and studied


Ngs has been able sequencing low-input dna of biologic specimens referenced to forensics, especially for mass disasters. this method can simultaneously analyze the low-input dna with different genetic markers including strs, snps, mutations and ect. therefore, the benefits of ngs technology include high efficiency, simultaneous detection of a large number of strs in autosomal and sexual chromosomes, the ability to detect alleles with similar length, and ect.


Although the high cost is a limitetion ngs. certainly due to the technical advances in this field and the ongoing efforts of genetic scientists, ngs will soon be utilized as a practical application and a common technology in various clinical trials as well as legal cases in genetic labs.


Ngs, dna typing, mass disasters, low-input dna