Investigating the protective effect of garlic on motility and sperm count in gamma-ray gamma rats
Abolfazl Ghafouri khosroshahi
,1,* Leila mousavi
,2 Maryam alvandi
1. Department of Medicinal Chemistry, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
2. Hamedan Province, Razan, Valiasr Hospital, Razan Department of Pathology
3. Hamedan University of Medical Sciences, Faculty of Medicine, Nuclear Medicine Group
Testicular tissue is an important organ of the male reproductive system, which is susceptible to ionizing radiation due to the presence of germ cells and disturbs the spermatogenesis. studies show that compounds with antioxidant properties can be effective in controlling oxidative stress, and in this regard, natural materials extracted from plants are effective because of their antioxidant properties and the absorption of free radicals produced by gamma rays. therefore, we used a herb that, in addition to the antioxidant properties mentioned, has a high daily intake in hamedan province.
First, we prepared garlic from a company specializing in the cultivation and domestication of medicinal plants. in dry shade conditions, to prepare the hydroalcoholic extract, the dried peppers were converted into finely divided pieces and then by using soaking methods. (maceration) and ultrasonic extracts were extracted. the rotary concentrate was then concentrated and the extract was stored in the refrigerator for biological tests.
experimental animal: in this study, male rats were used. they were kept in controlled conditions for 7-10 days in order to adapt to the environment. due to the extreme importance of temperature in this test, throughout the duration of the test, two thermometers showed temperature and all experiments were carried out in the range of 20 to 22 degrees. to create a 12-hour cycle of day and night, a laboratory timer is used to turn on and off the light source of the storage environment. all windows were also blocked to prevent light from entering.
conditions for exposure to gamma rays: the rats were divided into 5 groups of 8. the first group was selected as negative control and the second group was considered as positive control only by radiation and the groups treated with pepper extract (after determining the effective concentrations), three groups of mice exposed to gamma rays and also the specified concentrations of extract peppermint (100, 200 and 300) mg / kg was injected intraperitoneally and the groups treated with herbal extracts were injected 1 hour before exposure to gamma rays with 1.5 g of gamma rays. after six weeks, mice in all groups were dissected and the sperms were washed from the testicular tissue by hamsf10 and collected in 2 cm microtubes for sperm analysis. the testis of the testis isolated for pathological examination was sent to the pathology and sperm analysis laboratory.
Garlic hydroalcoholic extract at concentrations of 300 mg / kg showed a significant inhibitory effect on gamma-ray complications compared to the control group and increased the parameters of motility and number of spermatozoa
Hydroalcoholic extract of garlic is due to the presence of sulfide compounds capable of controlling and eliminating free radicals and inhibiting the degradation effects of the radicals formed on the motility parameters and number of sperm mice.
Herbal extracts, mouse sperm, garlic.