1. Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran 2. Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran
Extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae are an increasing concern regarding antibiotic resistance and their potential to cause serious infections which are difficult to treat. the purpose of this study was to assess an optimal strategy for the effective antibiotic treatment of urinary tract infections.
In this study, 90 isolates of esbl-e. coli isolated from patients with urinary tract infections were identified via biochemical and bacteriological methods. then, the antibacterial activities of vancomycin and trimethoprim were evaluated in vitro alone and in combination using disk diffusion agar and broth microdilution tests according to clsi document m100-s23 for bacteria. escherichia coli atcc 25922 as a reference quality-control strains was employed.
The mics range of vancomycin and trimethoprim against esbl-e. coli were 8-256 and 1-64 μg/ml, respectively, while for vancomycin in combination with trimethoprim was ranged from 0.128 to 8 μg/ml. indeed, the combination therapy of vancomycin/trimethoprim was exerted the synergistic effects in esbls producing isolates with fractional inhibitory concentration index ranged from 0.281 to 1.5.
Our present study demonstrates that vancomycin/trimethoprim combinations were effective in the treatment of utis with a higher efficiency. due to high risk factor of the β-lactamase producing isolates in uti infections, further studies are suggested to prevent their spread in community.