An association study between tnf-a polymorphisms and increased risk of prostate cancer

Saeideh Alidoost,1 Mohsen habibi,2 Farkhondeh pouresmaeili,3,* Eznollah azargashb,4

1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2. Central Laboratory, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran- Iran
3. Infertility and Reproductive Health Research Center (IRHRC), Shahid Beheshti University of Medical Sciences, Tehran, Ira
4. Department of Social Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Introduction

Prostate cancer, the second most common cancer in the overall men and the third in iranian men, and benign prostate hyperplasia (bph) are heterogeneous disorders with high prevalence among men. we are looking for new biomarkers enable us to detect the disease at early stages. tumor necrosis factor-alpha (tnf-α) polymorphisms may play any role in controlling the progression of prostate cancer, with change in expression level. the purpose of this study was to investigate the relationship between tnf-α-308g>a (rs1800629) polymorphism and prostate cancer.

Methods

In this case-control study, 320 peripheral blood samples including 100 pca patients samples, 110 samples from bph patients and 110 samples from healthy individuals were recruited from shohada-e-tajrish hospital, tehran- iran. all participants provided written consent and a personal questionnaire covered age, smoking consumption, body mass index (bmi), psa level, family history of cancer and detailed medical history. genomic dna was extracted by salting-out method, the tnf-α promoter rs1800629 snp was analyzed by means of specific primers and tetra-arms pcr, the products were analyzed on 2% agarose gel and the genotypes were determined based on the presence or absence of the desired bands. chi-square (χ2) tests was applied to assess statistically significant differences between subjects. binary logistic regression analysis was used to compute the odd ratios (ors) with 95% confidence intervals (cis). deviation of the genotype frequency from the hardy-weinberg equilibrium was tested using chi- square analysis. p-values of less than 0.05 were considered as statistically significant. all statistical analysis were done using ibm spss statistics version 25.

Results

In the present study, demographic characteristics of all three groups were studied. the relationship between smoking and genotypes gg and ga in rs1800629 showed there was no significant difference between bph and normal groups, while in the cancer groups there was a significant difference between the two genotypes (p=0.001). there was no significant difference between the two genotypes regarding other traits (p<0.05).

Conclusion

Our results suggest that tnf-α-308g/a (rs1800629) polymorphism could be used as a putative biomarker for early diagnosis of prostate cancer. however, this suggestion requires further studies of a larger population.

Keywords

Tumor necrosis factor-alpha, prostate cancer, benign prostate hyperplasia, rs1800629, polymorphism