1. Department of Laboratory Sciences, School of paramedicine, Shahid Sadoughi university of Medical Sciences 2. Department of Laboratory Sciences, School of paramedicine, Shahid Sadoughi university of Medical Sciences 3. department of biochemistry, school of medicine, shahid sadoughi university of medical sciences(international campus) 4. Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences
Abstract
Introduction
Nowadays cancer is the most important cause of mortality in human societies. lung cancer is the leading cause of death in men and the second one in women who suffered from cancer. doxorubicin is an anti-cancer drug that can be used in chemotherapy but it has some side effects such as cardiac-arrhythmia. it has been proven that nanoscience can increase the effectiveness of treatment while reducing the side effects of drugs. nanoliposome is one of these nanoparticles that can be used in drug delivery. the purpose of this research is to synthesize nano system containing chemotherapy drug with the aim of affecting lung cancer a549 cell line.
Methods
Nanoliposomes containing spc, cholesterol, brij76, dsp-mpeg(2000) and doxorubicin synthesized by thin-film hydration method. entrapment efficiency(ee) is measured with spectrophotometer and also drug controlled-release from nanoparticles is evaluated by dialysis method. the average of size and zeta potential of nanoparticles are assessed with dls.
Results
Doxorubicin encapsulation in liposomes was more than 85% and the drug release rate from nanocarriers showed a controlled and time dependent profile that was 38% in 48h in normal cell situation (37oc and ph:7.4) and 74% in 48h in cancer cell situation (42oc and ph:5.4). the mean diameter of nanoliposomes was smaller than 150nm and the zeta potential of them was negative.
Conclusion
In this study, a significant amount of doxorubicin was encapsulated in liposomes. drug release from nanocarriers showed a slow and continues kinetics. liposomes improve the solubility and bioavailability of the drug for delivering to lung cancer cells. anionic potential indicates that the system is suitable for the cancer cells.
