The association of sperm dfi with growth factors in fallopian tube

Seyed omidreza Mousavi,1 Roudabeh mohammadi,2 Fatemehsadat amjadi,3 Samaneh aghajanpour,4 Maryam eslami,5 Reza aflatoonian,6,*

1. 1-1. Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran. 1-2. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran
2. 2. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran
3. 2. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran
4. 2. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran
5. 1. Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
6. 2. Department of Endocrinology and Female infertility, Reproductive Biomedicine Research Center, Royan institute for Reproductive Biomedicine, Tehran, Iran

Abstract

Introduction

Fallopian tube is a part of female reproductive system in which fertilization, sperm preservation, capacitation and so on usually occurs. the female reproductive organs are some of the few adult tissues that reveal regular intervals of rapid growth. as with many other tissues, different growth factors like vascular endothelial growth factors (vegfs), fibroblast growth factors (fgfs), heparin-binding egf-like growth factor (hb-egf) and epidermal growth factor (egf) seem to have major effect on the female reproductive tract growth particularly fallopian tubes. the aim of our study is to investigate the influence of sperm dfi on the expression of different growth factors.

Methods

In this study sperm samples from 20 donors with normal features were collected and classified to two groups of normal and abnormal dfi. the third group was fallopian cells without sperm. finally, sperms were co-incubated with fallopian cells for 24h. to evaluate the expression of mentioned growth factors, rna was extracted from cells of different groups, cdna was synthesized and reverse transcriptase polymerase chain reaction (rt-pcr) was performed on the prepared cdna samples with the use of primers designed for vegf, egf, fgf2, hbegf. finally the pcr products were run on % 1.7 gel electrophoresis.

Results

Data analysis indicate that the expression of different growth factors in abnormal dfi group was the lowest and in normal sperm group was lower than the control. however, for accurate investigation more studies should be done.

Conclusion

This study has shown that abnormal dfi can reduce the expression of some growth factors which have essential roles in sperm preservation and fertilization. therefore, this hypothesis might be true that decreased expression of growth factors can disrupt fertilization events.

Keywords

Growth factors, fallopian tube, sperm, dfi