Lack of association between interleukin-34 single nucleotide polymorphism (rs3813904) and hepatitis b virus chronic infection

Nafiseh Mohammadi lamolang,1,* Seyed reza mohebbi ,2 Masoud parsania,3 Shabnam kazemian,4 Mahsa saeedi niasar,5 Mohammad reza zali,6

1. Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
2. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences
3. Department of Microbiology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
4. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences
5. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences

Abstract

Introduction

Chronic hepatitis b virus (hbv) infection is one of the main reasons of liver cirrhosis, hepatocellular carcinoma and high mortality in the world. the host genetic variations including single nucleotide polymorphisms (snps) are important factor in progression of hbv infection to chronic disease or clearance of the viral infection. the cytokines genes can affect the host immune responses and recent studies revealed a possible association between genetic variation in these genes and chronicity of hbv infection. il-34 that is a novel cytokine plays a probable role in the inhibition of hbv replication. the aim of present study was detection of a possible link between one snp (rs3813904) in il-34 gene and chronic hepatitis b infection.

Methods

In this study we choose 226 individuals (121 chronic patients and 105 healthy control). then genomic dna extracted of blood samples through salting out method. the snp was genotyped using the polymerase chain reaction restriction fragment length polymorphism (pcr-rflp) method.

Results

The frequency of (rs3813904 g/c) snp for gg, gc, cc genotypes were found 19.01, 47.93, 33.06 percent in the chronic patients and 22.85, 50.48, 26.67 in the control group percent respectively. results showed there is no significant difference among genotype frequencies p=0.539).

Conclusion

In this study, we didn’t find a significant link between the il-32 snp (rs3813904) and chronic hepatitis b infection. thus, it can’t be considered as a susceptibility factor for hbv chronicity.

Keywords

Hepatitis b, cytokine, il-34, single nucleotide polymorphism