Cymophenol (c6h3(ch3)(oh)c3h7) modifies neutrophil related inflammation in dermal injury
,1,* Hamed shoorei
,2 Hooman ravaei
,3 Parsa khalafi
,4 Danial gholamin
,5 Abbas majdi seghinsara
1. Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2. Department of Anatomical Science, Faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran.
3. Young Researchers and Elite Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran.
4. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
5. School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran
6. Department of Anatomical Science, Faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran.
2-methyl-5-isopropylphenol that also known as carvacrol or cymophenol, is a monoterpenoid phenol and a component of origanum vulgare (oregano) essential oil. studies have demonstrated that carvacrol have tissue protecting and inflammation suppressing effects. researchers have demonstrated that carvacrol could suppress cox-2 which is involved in prostaglandin biosynthesis of which has an important role in inflammation. it has also declared that it could inhibited prostaglandin synthesis.
in the other hand, inflammation and inflammatory reactions are marked as an important issue in skin wound healing. neutrophil-related inflammation results in an increase of myeloperoxidase production. mpo as a lysosomal protein that is stored in azurophilic granules, release into the extracellular space during neutrophil degranulation, and produces hocl from h2o2 and cl−. mpo oxidizes tyrosine to tyrosyl radical which is an oxidizing agent. all of these products are cytotoxic and may cause oxidative damage in host tissue.
in this study we administrated cymophenol to investigate its effects on neutrophil-related inflammation.
30 sprauge-dawley rats were divided in three groups. sham group, just with skin cutting; control group, with skin cutting and elevation of skin; cymophenol group, with skin cutting, elevation of skin, and cymophenol administration with 50 mg/kg dose. all skin cutting sizes and procedures were according to mcfarlane method. one week after, myeloperoxidase was measured in skin tissue to find out neutrophil activity.
In control group we observed significant mpo increase in comparison to sham group (p<0.01), but in the cymophenol group mpo activity was significantly decreased to 1.374±0.219 (p<0.05 vs. control group).
Our results show that cymophenol administration could decrease myeloperoxidase that could be considered as a marker for neutrophil-related inflammation and this issue represents that less oxidative stress occurs in host tissue. so it could be told that we would have decreased tissue damage and faster wound healing after skin injury. finally, we declare that cymophenol could be useful to suppress oxidative skin damage, but further studies are needed to clarify all aspects of this issue.
Cymophenol, neutrophil, inflammation, wound healing