1. Department of genetic, Tabriz branch, Islamic Azad University, Tabriz, Iran 2. Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Lung cancer is the main origin of mortality among cancers with less than 5 years survival rate for both men and women worldwide. kras and nf-ĸb signaling pathways have a critical role in the proliferation and progression of various cancers, including lung cancer. new theraputic approaches for lung cancer treatment is to target oncogenes like kras, map kinase, and nf-κb to disrupt signaling pathways related to proliferation and growth of cancer cells. kras mutation is a negative factor in various cancers including nsclc and complicate the therapeutic approaches with adjuvant chemotherapy. this research dedicated to analysis the effects of nf-κb inhabitor (ikk-2) and kras sirna in lung adenocarcinoma cancer cells proliferation and apoptosis.
the a549 lung cancer cells were treated with kras sirna and nf-κb inhibitor alone or in combination. the cytotoxic effects of kras sirna and nf-ĸb inhibitor were determined using mtt assay. relative kras and nf-ĸb mrna levels were measured by qrt-pcr. induction of apoptosis was measured by facs analysis.
The expression of mrna related to kras gene was reduced to 26.7%, respectively . also, mtt and facs assay results showed that treatment with kras sirna and nf-ĸb inhibitor results in reduction of the cell viability increase of apoptosis.
The results of this study indicated that kras and nf-ĸb signaling pathways might play an important role in the development and growth of lung cancer and might be a potential therapeutic target for treatment of lung cancer.