First time isolation of massilia timonae from eggshells in fars province

Susan Sohrabi,1 Nima bahador,2,*

1. Department of microbiology, College of Science, Shiraz Branch, Islamic Azad university, Shiraz, Iran
2. Department of microbiology, College of Science, Shiraz Branch, Islamic Azad university, Shiraz, Iran



Eggs with an average annual consumption of 250 per person per year are important foods in the diet of individuals. although egg is considered as an important food additive, it can be one of the causes of food poisoning. egg-level contamination occurs when it is formed in the chicken body when packaged or transmitted to the market. poisoning and common egg-induced contamination occur mainly due to the activity of salmonella, staphylococcus, and escherichia coli. because of the dangers of egg contamination and poisoning by it and its products, reducing the frequency and dose of contamination in these foods has been taken into consideration by manufacturers and health centers. the aim of this study was to determine the microbial contamination of eggshells collected from different factories in fars province and identify the most common isolates using molecular technique.


the eggs were collected from four companies. bacterial isolation was done using tsb and blood agar. in the next step, identification of the isolates was carried out using differential culture media, gram staining, and biochemical tests. the most common isolates were examined by pcr. in the last stage, antibiotic susceptibility was tested to determine the sensitivity and antibiotic resistance of the bacteria.


for the first time massilia timonae were detected from eggshells. the organism were susceptible to most of the antibiotics, while it was resistant to oxacilline.


because of the nutritional value and high consumption of eggs in different countries, the packaging is different according to the conditions and location of the storage, transportation and packaging systems. as a result, the organisms are different, which is one of the factors involved in the pathogenicity process. therefore, increasing the awareness of the personnel working in the field of egg packing and distribution, the cleaning of related systems can help to reduce the number of microorganisms and consequently reduce pathogenicity.


microbial contamination, eggshell, massilia timonae