Investigation of antibiotic resistance pattern and phenotypic diagnosis of extended-spectrum β-lactamase enzymes in the isolated klebsiella pneumonia strains of pulmonary patients admitted in shoml sp

Ali Khosravi,1,* Fateme zaboli,2 Ismail fattahi,3

1. Islamic Azad University of Ayatollah Amoli
2. Islamic Azad University of Ayatollah Amoli
3. Islamic Azad University of Ayatollah Amoli



One of the antibiotic resistance mechanisms in the klebsiella pneumonia is produce of extended- spectrum β-lactamase (esbl) enzyme. encoding genes of esbl are usually located on the plasmid and can be transmitted to other strains with negative grams. this study aimed to examine the frequency of klebsiella pneumonia producing bacteria of esbl.


The present descriptive-cross sectional study have done on 20 strains of klebsiella pneumonia isolated from exferimental (tria) samples of patients referred to shomal specialized hospital of amol in 2018, and identified by biochemical-differential tests. in order to evaluate the antibiotic resistance pattern, disc diffusion method was used, and organism producing esbl identified by doing verified screeing and phenotypic tests


According to results of this study, the most relevant antibiotic resistance relates to antibiotics cefotaxime (95 %), ceftriaxone (95 %) and imipenem (70 %) and the lowest to amikacin (40 %) and gentamicine (40 %). also 14 positive strains of esbl klebsiella pneumonia were identified.


High prevalence of antibiotic resistance and production of esbls in the studied area show the need for screeing clinical samples concerning esbls by the laboratory and using appropriate antibiotics with β-lactamase and antibiotic inhibition capability as combined with clavulanic by doctors.


Klebsiella pneumoniae, antibiotic resistance, esbl