1. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar 2. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar
recurrent miscarriage is a common problem. recurrent miscarriage is determined as the loss of a gestation before 20 weeks. many markers have been introduced in the recurrent miscarriage. one of these markers is the gene encoding the leptin receptor (lepr) protein. the lepr protein categorized as a interleukin6 from the family of type1 cytokines with six isoforms: lepra, b, c, d, e, and leprf gene is one of the major genes on the biological pathway of obesity, before study show that expression of lepra and leprb are effective in stimulating of lh in the rat ovaries
To investigate the correlation of rs1137100 (511a>g) which is located on exon 6 of lepr with the risk of recurrent miscarriage in the iranian womens population.dna extraction and the sscp have been used for the blood sample of 70 patients and 70 controls participants
Based on the obtained from sscp gel analysis frequency of genotypes that obtained in the controls were 22.8% gg, 51.4% ag and 25.7% aa, and in the cases were 12.8% gg, 61.42% ag and 25.7% aa. also, allelic frequency that calculated in controls were 51.4 and 48.5 percent for a and g allele, and in cases were 56.4 and 43.5 percent for a and g allele. statistical analysis revealed that the distribution of genotypes for 511a>g transition does show significant different (p = 0.36, or=1.26; 95% ci: 0.76-2.1) in case and control groups
Based on, we recommend that, the 511a>g- lepr gene transversion may be a genetic risk factor for idiopathic recurrent miscarriage in the mazandarn female population.