1. 1- Molecular and Medicine Research Center, Department of Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran 2. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine,Next to Milad To
Abstract
Introduction
Gastric cancer (gc) is among the most common cancer types in the world and one of the most lethal gastrointestinal cancers. micrornas (mirnas) can be of great importance in the early detection of gc. this study aimed to investigate some mirnas compared to the control group.
Methods
Total rna was extracted from the paraffin tissue of gc patients and healthy volunteers. the expression levels of mirna – 17, mirna - 25 and mirna -133 were assessed using real time rt-pcr with specific primers
Results
Our data showed that mirna – 17, mirna - 25 were up -regulated in gc patients compared to the control group. mir-133b expreesion was not different between patients and control group.
Conclusion
It appears that the expression of mirnas and genes expression pattern in iranian patients with gastric cancer are similar to the other populations. we can use these mirnas as a strong tool for the diagnosis of gc in the early stages.
