Identification micrornas13-17 and 133 as biomarkers non-invasive diagnosis of circulating and prognostic marker in patients with gastric cancer

Parisa Zia sarabi,1 Faezeh ghasemi,2,*

1. 1- Molecular and Medicine Research Center, Department of Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
2. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine,Next to Milad To



Gastric cancer (gc) is among the most common cancer types in the world and one of the most lethal gastrointestinal cancers. micrornas (mirnas) can be of great importance in the early detection of gc. this study aimed to investigate some mirnas compared to the control group.


Total rna was extracted from the paraffin tissue of gc patients and healthy volunteers. the expression levels of mirna – 17, mirna - 25 and mirna -133 were assessed using real time rt-pcr with specific primers


Our data showed that mirna – 17, mirna - 25 were up -regulated in gc patients compared to the control group. mir-133b expreesion was not different between patients and control group.


It appears that the expression of mirnas and genes expression pattern in iranian patients with gastric cancer are similar to the other populations. we can use these mirnas as a strong tool for the diagnosis of gc in the early stages.


Micro rna, gastric cancer, helicobacter pylori, rt-pcr