1. Medical science of islamic Azad University,Ardabil &Shahid Beheshti University of Medical Science 2. Mohageg Ardabili University
Abstract
Introduction
Cancer is regarded as a complex disease which is non-curable.ttraditional medicines have been applied for the treatment of cancers in the world. prangos pabulariadc.(apiaceae) is a perennial herb native to mountain slopes of the central and western asia countries. prangos species are wiedely used in folk medicine. anticancer activity of prangos pabularia extract against hela line was studied using cytotoxicity effect. many antitumor agents, have been reported to induce apoptotic cell death. this cell death plays a critical role in killing tumor cells in cancer therapy
Methods
In order to evaluate of cytotoxicity and antioxidant properties of the plant, dried powdered of roots of p.pabularia were soxhlet extracted successively with n-hexan, dichloromethane (dcm) and methanol. viability and cytotoxicity of hela cell line were measured by mtt assays. the antioxidant potential of the plant extracts was evaluated by dpph assay. additionally characteristic of cell death were using h&e morphological staining.
Results
The dcm extract showed a significant antioxidant effect with rc50 value of 0.08 mg/ml and our results showed that dcm extract displayed a significant cytotoxic activity against hela cell line in culture with a ic50 value of 0.526 mg/ml in 24h with mtt assay. additionally characteristic of aponecrotic cell death confirmed using h&e morphological staining
Conclusion
This study reveals biological effects and antitumor activity of prangos pabulariadc.(apiaceae) extract against hela line.
