Schwann cells growth characterization on electrospun nanofibrous scaffolds for neural tissue engineering

Yeganeh Hazeri,1 Shiva irani,2,* Mojgan zandi,3 Mohammad pezeshki moddares,4

1. Biology Department, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2. Biology Department, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3. 3. Department of Biomaterials, Iran polymer and Petrochemical Institute, Tehran, Iran
4. Burn Research Center, Iran University of Medical Science, Tehran, Iran

Abstract

Introduction

The regeneration and repair of peripheral nervous system (pns) are important global clinical problems. many approaches for nerve therapy have been used. different surgical methods like, end to end surgery is more common for nervous injuries, but it is can be useful when the gap is small and also another problems like losing function and couple surgeries are exist. according to entries mentioned tissue engineering may act as a gold technique for neural repair. synthetic, natural and sometimes mix of both of them can be used for tissue engineering applications. in this paper polyvinyl alcohol and alginate sulfated has been used via electrospinning with different percentages (90/10-80/20 wt %).

Methods

Schwann cells were purchased from stem cell technology research center. the cells routinely cultured in t-25 flask with 10% fetal bovine serum and dmem. at passage 3 seeded into 25 well plates for mtt assay during 7 days. then 0.15 mg/ml was added to each well in different days, incubated for 4 hours in 37 ᵒc with 5% co2 in dmem medium. the formazan crystals dissolved in 200 µl of dimethyl sulfoxide (dmso). then absorbance was read at 490nm via a spectrophotometric plate reader. sem technique was used for study surface and morphology of nanofibrous scaffolds.

Results

Figure (1) is sem images for morphology of scaffolds that shows nanofibers are random mats and are suitable for neural tissue engineering. figure (2) is mtt assay that demonstrate that scaffolds have no cytotoxicity and cells had survivality and also showed that the cell viability significantly improved (p<0.001) during 7 days.

Conclusion

Nanofibers scaffolds of pva/alginate sulfated can support cell attachment and proliferation of schwann cells during this study. this paper confirmed the effective roll of this electrospun scaffolds for nerve tissue engineering.

Keywords

Tissue engineering, electrospinning, polyvinyl alcohol, alginate sulfated