Effects of sal-like 4 sirna on proliferation and apoptosis on breast cancer

Seyede atefe Hosseini,1 Amirreza hesari,2 Soheila moeini,3 Faezeh ghasemi,4 Rezvan mohammadi,5 Reza salarinia,6,*

1. MSc student of Medical Biotechnology, School of Medicine, Northern Khorasan University of Medical Sciences, Northern Kh
2. Department of Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
3. Mashhad University of Medical Sciences, Mashhad, Iran
4. Department of Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
5. MSc student of Medical Biotechnology, School of Medicine, Northern Khorasan University of Medical Sciences, Northern Kh
6. Department of Medical Biotechnology and Molecular Sciences, School of Medicine, North Khorasan University of Medical Sci

Abstract

Introduction

Breast cancer is found as the most cancer among women worldwide. it has been showed that a variety of cellular and molecular pathways involved in initiation and progression of breast cancer. spalt like transcription factor 4 is known as a transcription factor that has critical roles in the proliferation of cancerous cells. in the current study, we assessed the effect of sal-like 4 sirna on the proliferation of mcf7.

Methods

We were cultured mcf7 cell lines and transfected it by a specific sall4 sirna. we determined toxic doses of sall4 sirna . we were obtained expression levels of the sall4 and bcl-2 gene by real time-pcr method. moreover, cell death was evaluated by pi staining and facs analysis.

Results

The specific concentration of sall 4- sirna was 62.5 nmole. the gene expression analysis showed that an expression level of bcl-2 gene in the sirna group was significantly decreased. cell cycle results in the group treated with sirna and the cell control group showed that the treated group had a higher percentage of subg1 (24%) than control group of cells (0.65%). subg1 percent represents apoptosis in the cell (p <0.05).

Conclusion

Sirna could increase apoptosis of breast cancer cells via reducing expression of bcl-2 and sall 4. hence, it seems that these genes could be used as novel options for targeted therapy. this strategy could increase the specificity of drug, and is able to reduce the side effects than conventional chemotherapy in in vivo models.

Keywords

Breast cancer, sall4 , sirna , bcl-2