1. Department of Microbiology – shahr-e-Qods Branch – Islamic Azad University –Tehran / Iran 2. Department of Microbiology – shahr-e-Qods Branch – Islamic Azad University –Iranian Gene Fanavar Institute (IGF), Tehran 3. Department of Microbiology – shahr-e-Qods Branch – Islamic Azad University –Tehran / Iran
Abstract
Introduction
Contamination of biological products and cell cultures is one of the important problems in producing a good and safe product for use. rapid diagnosis contaminant agents are very important and vital in producer facility.
one of the most important contamination factors is acholeplasma laidlawii, which is commonly found in bovine serum, which is the basis of many cell culture and another medium. polymerase chain reaction is a fast, sensitive and high-specific technique for detecting contaminants. the aim of this study detect acholeplasma laidlawii contamination in biological products by pcr method.
Methods
Pcr test optimized by standard strain of acholeplasma laidlawii dna.then specificity and limit of detection (lod) evaluated by standard methods.a total of 100 positive pplo-broth samples were collected from razi institute. dna was extracted by dng plus and pcr test was performed on samples.
Results
505 bp amplicon was detected in 1.5 % agarose gel electrophoresis. in specificity test, acholeplasma laidlawii just observed and none of other organisms were positive. from100 samples accessed by pcr, 38 (38%) were obtained positive.
Conclusion
According to studies, it has been determined that the pcr method is a suitable and accurate method for the detection of acholeplasma laidlawii contamination, and rapid detection of this bacteria is important for production of biological products.
