Investigating the expression alteration of long non-coding rna gnas-as1 of breast cancer specimen

Hesam Motaleb zade,1,* Seyed javad mowla,2 Shiva irani,3 Manijeh motevalian,4

1. Department of biology, Faculty of Basic Sciences Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
3. Associate Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4. Razi Drug Research Center (RDRC), Iran University of Medical Sciences (IUMS), Tehran, Iran

Abstract

Introduction

Breast cancer is the most common cancer in women, and due to high rates of invasion and metastasis in these patients, the first cause of death is in women aged 40-44 years. the latest bio markers and prognostic factors in a variety of cancers, including breast cancer, can be found in the form of long non-coding rnas with a variety of oncogenic function or tumorigenic function (tsg), a marked change in the expression of this cancer showing normal breast tissue. the aim of this study was to simultaneously investigate changes in the expression of gnas-as1 non-coding gene expression in tumor tissue of breast cancer.

Methods

Mcf7 and mcf10a cancer cell lines were used to optimize the experiments. then with the method qrt-pcr the expression of a non-coding rna gene, gnas-as1 was investigated in 45 pairs of tumor and adjacent tumor fresh samples.

Results

According to the results of the study, 90 tumor samples of breast cancer were compared to the normal tumor margin of the same person, non-coding rna of gnas-as1 have a pronounced increase in tumor tissue expression relative to the tumor margin (p = 0.0453 gnas-as1).

Conclusion

Considering the review of previous articles and micro array studies in human specimens, the existence of a possible hypothesis about the oncogenesis of the selected gene in the present study has been found based on real-time pcr results and comparison of data, gnas-as1 gene increased significantly in the cancer cell line compared to the normal cell line and in the cancerous tissue compared to normal tissue which resulted in the probability of proto-oncogene of this gene.

Keywords

Gene- breast- oncogene- tsg- long non-coding rnas -term - real-time pcr