Identification of bacterial agents of urinary tract infection in patients referred to a laboratory during six months

Roozbeh Yalfani,1,* Marzyeh pazoki,2

1. Academic member - Nursing Department - Faculty of Medical Science - Islamic Azad University - Varamin Pishva branch
2. Student of Microbiology - Microbiology Department - Faculty of Science - Islamic Azad University - Varamin Pishva branch



Establishing a diagnosis of symptomatic uti requires a patient to have symptoms and signs of a uti and laboratory tests confirming the diagnosis. although several different microorganisms can cause utis, including fungi and viruses, bacteria are the major causative organisms and are responsible for more than 95% of uti cases. the aim of this study is identification of bacterial agents caused uti within winter 95 - spring 96.


This study was performed in a laboratory located on pakdasht. a total of 3136 urine specimens were collected consecutively within a period of six months. patient ranged from 14 days to 68 years old. adult patients were sampled by clean catch midstream urine and children aged under 3 years were sampled using sterile urine bags. the specimens were inoculated onto nutrient agar plates. inoculated plates were then incubated aerobically at 37°c for 24 hours. after 24 hours, discrete colonies were picked up and gram stained. further sub-culturing for gram negative bacilli was done on eosin methylene blue agar to obtain a pure culture for biochemical tests.


3136 urine samples were analyzed, of which 130 (4.14%) had significant bacteriuria (≥105 cfu/ml and pyuria ≥10 white blood cells/high-powered field). the rate of positive culture was 4.47% (89/1990) for female subjects and 3.57% (41/1146) for male subjects.


Analysis of the results according to patient gender indicated that although e. coli is the predominant isolated pathogen from both sexes, it occurred more frequently in women.


Uti - bacterial agent - e. coli