Comparative mir34a expression profile analysis in tumor and normal tissues of patients with colorectal cancer

Mahsa Rafieian,1,* Doctor seyed reza kazemi nezhad,2 Doctor mohamad reza hajjari,3 Maryam tahmasebi birgani,4



Colorectal cancer is a type of prevalent cancers in populations and allocates numbers of mortality. recently, researchers noticed to noncoding rnas as interventionist factors to develop cancers. micrornas is one of the noncoding rnas family that has an important role in cell biology processes like growth, proliferation, differentiation and apoptosis through post-transcriptional regulation of gene expression. mirnas regulate expression of some oncogenes and tumor suppressor genes and mirna expression altered reported in large numbers of cancers. mir-34a induces cellular apoptosis and cell cycle suppression in g1 phase and their expression reduces in some cancers.


This study examined 10 pairs of tissues of the patients with colorectal cancer. after collection, sample tissues and extracted total cellular rnas, we synthesized cdna by stem-loop rt-pcr. the expression of mirna34a measured using real time pcr assey.


However, the results showed reduction expression of this mirna in tumor tissues in comparison of normal tissues.


These results thus suggeste that the overexpression of mir34a be associated with the development of colorectal cancer, meanwhile mir34a may be involved in the development and progression of colorectal cancer mirna34a is a tumor suppressor factor that is so important for diagnose and treatment of colorectal cancer.


Colorectal cancer, mirna34a, stem-loop, rt-pcr