1. islamic azad university east tehran branch 2. islamic azad university east tehran branch
Abstract
Introduction
Gastric cancer is the fifth leading cause of cancer and is also the third leading cause of death in the world .although the molecular mechanisms of carcinogenesis of the stomach are still unclear, recent epigenetic extinction of tumor-associated genes has been shown to be a tumorigenic mechanism by hypermethylation of the promoter. the paired box gene (pax5) encodes a family of regulator transcription factors that performs important central functions in cell differentiation and tissue development. research has shown that pax5 is a very important tumor suppressor gene in gastric cancer. the aim of this study was to investigate the methylation status of promoter of pax5 gene in blood samples of people with gastric cancer and normal people and to investigate their role as prognostic and diagnostic factors in gastric cancer.
Methods
In this study, the methylation status of pax5 promoter was evaluated using ms pcr technique in 35 blood samples of gastric cancer patients and 15 blood samples of normal people in iranian population.
Results
The results showed that 27% of the promoter of the pax5 gene was methylated in the blood samples of patients with gastric cancer and all of the normal samples were unmethylated.
Conclusion
Investigating the increased methylation of the pax5 promoter in blood samples of people with gastric cancer compared with normal ones can be used as a biomarker for gastric cancer prognosis.
