Silibinin inhibits mir-17 in co-cultured huvecs with a549 cell line

Somayeh Mirzaaghaei,1 Ali mohammad foroughmand,2,* Ghasem saki,3 Mohammad shafiei,4

1. Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2. Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3. Cellular and Molecular Research Center, School of medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Ir
4. Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract


Introduction

Micrornas are involved in different biological processes including cell proliferation and apoptosis, the two intimately linked processes that critically play role in the development and progression of human malignancies. emerging experimental evidences have indicated that targeting mirnas can be a novel strategy for cancer prevention and/or therapy. using “natural agents” to target mirnas is one of the promising strategies that is known to contribute to processes of tumor development and progression. it has been revealed that silibinin, the major active constituent of seeds extract of silibum marinume, has anti-oxidant, anti-cancer, anti angiogenic, and anti-inflamatory effects. in this study, using a co-culture system, we have evaluated the effects of silibinin on gene-expression changes of mir-17 in human umbilical vein endothelial cells (huvecs). a549 cells were used as the inducer of angiogenic switch in huvecs.

Methods

Mtt assay and wound-healing migration assays were used to determine the best concentration of silibinin on co-cultured huvecs with a549 cells. real-time rt-pcr was used to assess gene-expression changes of intended microrna.

Results

Silibinin reduced mir-17 gene expression in huvecs co-cultured with a549 nearly to mir-17 gene-expression of simple cultured huvecs.

Conclusion

Silibinin has the potential to inhibit mir-17 in huvecs, and would be a promising agent in treatment strategies to inhibit tumor induced angiogenesis.

Keywords

Silibinin, mir-17, huvecs, tumor angiogenesis