Mortality effect of silibinin on the a549 cell line

Somayeh Hoseini,1,* Alimohmmad foroghmand,2 Mohammadreza hajjari,3 Somayeh mirzaaghaei,4



Lung cancer is the leading cause of cancer death in the world, with 1,375,000 deaths annually. common chemotherapy such as the use of cisplatin, depending on the source of the phenomenon, causes resistance in 63-80% of the cases, thus they have poor performance in the treatment of cancer. anti-cancer properties of herbal compounds have attracted widespread interest in new research. they are believed to have significantly contributed to the development of new and more effective and lower resistance anticancer therapies in comparison with chemotherapy drugs such as cisplatin silibinin is a flavonolignan which is extracted from milk thistle (silybum marianum l.). this herbal substance has an antitumor potential in treating many cancers, and in recent years has shown promising results against all types of cancer such as prostate, colon, bladder, lung, and ovary. in this study, the effect of cytotoxicity of silibinin in a549 cell line was examined


Cells were treated with different concentrations of silibinin (25, 50, 75, 100 and 125 μg /ml) for 24 hours, 48 hours and 72 hours and the viability of the cells was analyzed by mtt assay at different concentrations.


The viability of the a549 is observed in response to increasing doses of silibinin.


It can be concluded that silibinin has toxic effects on a549 cell line and can be used as a chemopreventive agent in treating strategies in lung cancer patients.


Silibilin,a549, mtt assay, lung cancer