1. Islamic Azad University,zanjan branch, faculty of baisic sciences, department of Genetics,Etemadeyeh.moalem st. 2. Islamic Azad University,zanjan branch, faculty of baisic sciences, department of Genetics,Etemadeyeh.moalem st.
Abstract
Introduction
Claudins are the main structural and functional proteins of tight junctions in epithelial cells. the altered expression patterns of differ¬ent claudin members have been demonstrated in a variety of diseases, particularly in cancers. it has been reported that claudin 1 is overexpressed 22-fold in gastric adenocarcinoma. considering the least side effect of herbal extracts, in this study we have evaluated claudin-1 gene expression in ags cells treated with crocus sativus extract.
Methods
The ags cells were incubated 37°c containing 5% co2 with 85% humidity dmem with 10% fbs . the cells were treated with concentrations of 800, 1200, 2000 μg /ml of crocus sativus for 48 hours. extraction of rna, synthesis of cdna has been done using kit. the study of claudin-1 gene expression was performed by real time pcr and also gapdh gene was used as the internal control.
Results
The results of real time pcr data shows significant decrease in expression rate of claudin1 gene in all concentration of crocus sativus . the most reduction was 85% in 2000 μg /ml.
Conclusion
Claudin-1 may become therapeutic targets for cancer treatment and crocus sativus as herbal extract decreased the expression rate of claudin1significantly. it seems that this herbal extract has tremendous anticancer effect.
