1. medical science of Shahid beheshti university 2. medical science of Shahid beheshti university 3. medical science of Shahid beheshti university
Abstract
Introduction
Acute myeloid leukemia (aml) is the most type of acute leukemia in adults. in solid tumors, alteration of energy metabolism in cancer cells has been proven. but in leukemia, studies in this area are limited. many metabolic pathways are active in the cell, including the tricarboxylic acid (tca) cycle. malate dehydrogenase 2 (mdh2) and isocytrate dehydrogenase 2 (idh2) are two key genes of tca cycle. the aim of this study was to evaluate the expression of idh2 and mdh2 genes in patients with acute myeloid leukemia.
Methods
Mdh2 and idh2 expression levels were measured by means of real-time polymerase chain reaction in 65 primary aml samples which then were compared to genes expression levels observed in 10 healthy subjects.in order to analysis of data spss 16 and graph pad prism 6.0 soft wares have been applied.
Results
The results showed that the relative expression of idh2, mdh2 genes in the patients group was significantly increased 5.91and 8.83, respectively (p value <0.0001 and p value <0.05 respectively).between the age of the patients and the expression of these genes significant correlation was found (p value: 0.002, r:-0.376 and p value<0.0001, r: 0.509 for mdh2 and idh2 respectively). also there was a significant relationship between the percentage of patients' blast and expression of mhd2 gene (p value 0.005, r:-0.343).
Conclusion
Increased expression of idh2, mdh2 in aml patient group in this study is in line with previous studies which described the alteration expression of metabolism involved genes in cancers.
