Interaction of rs783540 of cpeb1 gene in patients with azoospermia

Hosna sadat Zahed shekarabi,1,* Zahra noormohammadi,2 Iman salahshoori far,3 Naser kalhor,4

1. Department of Genetics, Faculty of Basic Sciences Science and Research Branch, Islamic Azad University Tehran, Iran
2. Associate professor, Science and Research Branch, Islamic Azad University, Tehran, Iran
3. Assistant professor, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction

: publicly, 90% of fertile couples get pregnant in one year. therefore, infertility refers to disability in pregnancy, after 12 months of regular intercourse, without the use of any contraceptive method. the purpose of the study was to investigate interaction of rs783540 of cpeb1 gene in patients with azoospermia.

Methods

Blood samples of 100 azoospermic men and 100 healthy men with at least one child and no history of a specific disease were obtained from the qom infertility center and their genomic dna was extracted by salting out. pcr-rflp technique was used to investigate snp polymorphism. chi-square tests and logistic regression of allele and genotype snp were evaluated in two healthy and healthy groups.

Results

According to the statistical analyzes performed on rs783540, it was found that the majority of allele a (ancestral) controls were greater (66%) and in the patient samples, the frequency of g alleles was higher (65%). the p value in the control and patient samples was 0.045 and 0.0001, respectively. the samples are not in the hardy weinberg equilibrium and have a significant relationship.

Conclusion

According to studies conducted and the high expression of cpeb1 in the testis, this gene was selected as the target gene. based on the results obtained in this study, the patient with a high g allele (65%) is likely to have a lower expression of cpeb1 in testicular tissue, which probably causes male infertility. according to studies conducted and the high expression of cpeb1 in the testis, this gene was selected as the target gene. based on the results obtained in this study, the patient with a high g allele (65%) is likely to have a lower expression of cpeb1 in testicular tissue, which probably causes male infertility.

Keywords

Male infertility, snp, cpeb1 gene