Investigation of cytotoxicity, cell cycle and induction of apoptosis by methyl-thiosemicarbazone complex with cu2+ in k562 cell line

Neda Kazemi motlagh,1,*

Abstract


Introduction

Thiosemicarbazone compounds have previously been considered as a new series of potent apoptosis-inducing agents. in the present study, we evaluated the influence of an active compound from methyl-thiosemicarbazone family on growth inhibition and induction of apoptosis in k562, chronic myeloid leukemia cells

Methods

The viability and growth inhibition of the treated cells were measured in various concentrations (10-120 μm) during times 24, 48 and 72 hours by mtt assay. results showed that the viability and proliferation decrease dose dependently at during 24-72 hrs. the ic50 value was determined as 80±3.0 μm. analysis by flow cytometry revealed sub-g1 cell cycle arrest in k562 cells treated with this compound

Results

Results showed that the viability and proliferation decrease dose dependently at during 24-72 hrs. the ic50 value was determined as 80±3.0 μm

Conclusion

Based on these data, it seems that methyl-thiosemicarbazone complex with cu2+can be considered as a good candidate for next pharmaceutical evaluations

Keywords

Apoptosis, chronic myeloid leukemia, thiosemicarbazone, k562 cells.